NGS Sample submission

BEA is preparing libraries as described but also accepting libraries prepared by the user. Importantly, the submitted sequencing libraries has to meet requirements and important information regarding the sequencing has to be stated and submitted. Therefore, users shall always indicate type of flowcell, general sequencing setup, indexes and eventual custom primers that should be used during sequencing. Information about library complexity and diversity are also important and should be communicated.

Libraries should be prepared in 10 nM concentration if possible. Libraries submitted to BEA has to be checked for concentration using fluorometric measurements (Qubit) and for quality Bioanalyzer (TapeStation).

We strongly recommend users to pool their samples before submission since BEA will generally not pool samples for sequencing. For pooling of multiple samples BEA will charge the cost for quality control for all individual samples.

Information and considerations about different options regarding different platforms and flowcells can be found in this RNA sequencing guide from Illumina.

General information for different types of chemistry and index sequencing on the Illumina systems can be found in this guide from Illumina.

When submitting your samples please consider following points:

  • Include a signed BEA submission order form with correct invoice address and KI ZZ code and account project.
  • Indicate type of sequencing and desired target concentration (pM) in the order form.
  • Indicate the sequence readout, single read (SR) or paired end read (PE) and number of cycles.
  • Include and send a sample sheet with the sample names together with information about the barcode index in the pools.
  • Sample sheet template can be downloaded here
  • Please note that the following characters are not allowed in the names:
    The space character and ? ( ) [ ] / \ = + < > : ; " ' , * ^ | & .