Methylation Analysis

BEA offers solutions for studies of DNA methylation on the epigenetic level with the Illumina Infinium MethylationEPIC v2.0 BeadChip.

Methylation profiling with Bead array technology allows researchers to analyze the effects of aberrant methylation (either hyper- or hypomethylation) for a variety of applications. Illumina has developed a robust methylation profiling platform that provides quantitative methylation measurement at the single-CpG-site level, providing the highest resolution for understanding epigenetic changes.

The Infinium MethylationEPIC 2.0 bead chip is the successor of the popular Infinium Methylation 450K bead chip. It covers >90% of CpGs found on Infinium Methylation450K plus an additional 350,000 CpGs in enhancer regions. These enhancer regions, identified by ENCODE and FANTOM5, have been shown to be critical sites for differential methylation.

There are no major changes upon the succession, apart from bead chip sample format, where Infinium Methylation EPIC bead chip is in 8-sample format and Infinium Methylation 450K bead chip is in 12-sample format. It uses the same robust chemistry and workflow as in Infinium Methylation 450K.

≥500 ng gDNA of good quality in 45 ul is recommended to use as input. Quantitative measurement should be performed by using a spectrofluorometer. Qualitative measurement is performed by using Agilent Tapestation genomic tapescreen.